Structure of the gene coding for the sequence-specific DNA-methyltransferase of theB. subtilisphage SPR | Zendy

György Pósfai | Zendy; F. Baldauf | Zendy; Sára Erdei | Zendy; János Pósfai | Zendy; Pál Venetianer | Zendy; Antal Kiss | Zendy

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Structure of the gene coding for the sequence-specific DNA-methyltransferase of theB. subtilisphage SPR

Author(s) -

György Pósfai,

F. Baldauf,

Sára Erdei,

János Pósfai,

Pál Venetianer,

Antal Kiss

Publication year - 1984

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/12.23.9039

Subject(s) - biology , bacillus subtilis , nucleic acid sequence , gene , genetics , coding region , dna methyltransferase , dna , microbiology and biotechnology , methyltransferase , homology (biology) , peptide sequence , repeated sequence , genome , bacteria , methylation

The nucleotide sequence of the gene coding for the 5'-GGCC and 5'-CCGG specific DNA methyltransferase of the Bacillus subtilis phage SPR was determined by the Maxam-Gilbert procedure. Transcriptional and translational signals of the sequence were assigned with the help of S1 mapping and translation in E. coli minicells. The gene codes for a 49 kd polypeptide. The amino acid sequence of the SPR methylase shows regions of homology with the sequence of the 5'-GGCC-specific BspRI modification methylase.

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