Open AccessStructure of the gene coding for the sequence-specific DNA-methyltransferase of theB. subtilisphage SPR
Author(s) -
György Pósfai,
F. Baldauf,
Sára Erdei,
János Pósfai,
Pál Venetianer,
Antal Kiss
Publication year - 1984
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/12.23.9039
Subject(s) - biology , bacillus subtilis , nucleic acid sequence , gene , genetics , coding region , dna methyltransferase , dna , microbiology and biotechnology , methyltransferase , homology (biology) , peptide sequence , repeated sequence , genome , bacteria , methylation
The nucleotide sequence of the gene coding for the 5'-GGCC and 5'-CCGG specific DNA methyltransferase of the Bacillus subtilis phage SPR was determined by the Maxam-Gilbert procedure. Transcriptional and translational signals of the sequence were assigned with the help of S1 mapping and translation in E. coli minicells. The gene codes for a 49 kd polypeptide. The amino acid sequence of the SPR methylase shows regions of homology with the sequence of the 5'-GGCC-specific BspRI modification methylase.