Identification ofXenopus laevismRNAs with homology to repetitive sequences | Zendy

Walter Reith | Zendy; Georges Spohr | Zendy

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Identification ofXenopus laevismRNAs with homology to repetitive sequences

Author(s) -

Walter Reith,

Georges Spohr

Publication year - 1984

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/12.23.8899

Subject(s) - biology , xenopus , complementary dna , messenger rna , translation (biology) , genetics , coding region , homology (biology) , microbiology and biotechnology , repeated sequence , gene , genome

Hybrid selection translation experiments have been carried out with genomic and cDNA relatives of two repetitive sequence families. On the basis of the in vitro translation products detected, it was found that transcripts complementary to these repeats are linked to several different mature mRNAs in stage 40 embryos of Xenopus laevis. One repeat hybridizes to mRNAs that direct the synthesis of 17 proteins. The second is present on mRNAs coding for 3 proteins. By estimating the abundance of these proteins among the translation products of total embryonic mRNA, it is inferred that all of the repeat bearing mRNAs are rare, less than one in 20,000 mRNA molecules.

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