Polymer supported DNA synthesis using hydroxybenzotriazole activated pbosphotriester intermediates | Zendy

J. E. MARUGG | Zendy; Norbert Piel | Zendy; Larry W. McLaughlin | Zendy; M. Tromp | Zendy; G.H. Veeneman | Zendy; G.A. van der Marel | Zendy; Jacques H. van Boom | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Polymer supported DNA synthesis using hydroxybenzotriazole activated pbosphotriester intermediates

Author(s) -

J. E. MARUGG,

Norbert Piel,

Larry W. McLaughlin,

M. Tromp,

G.H. Veeneman,

G.A. van der Marel,

Jacques H. van Boom

Publication year - 1984

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/12.22.8639

Subject(s) - dna , biology , phosphate , dna synthesis , phosphorylation , polymer , biochemistry , combinatorial chemistry , organic chemistry , chemistry

Phosphorylation of N-acyl-5'-O-DMTr-d-nucleosides, or similarly protected DNA-dimers having a free 3'-OH group, with 2-chlorophenyl-0,0-bis(1-benzotriazolyl)phosphate affords reactive 3'-phosphotriester derivatives. The latter intermediates can be used, without further purification, for the synthesis of DNA-fragments on the controlled pore glass/long chain alkylamine support. Further, 2-cyano-1,1-dimethylethoxy dichlorophosphine proved to be very suitable for the preparation of 5'-phosphorylated DNA-fragments on the same type of solid support.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research