Open AccessPolymer supported DNA synthesis using hydroxybenzotriazole activated pbosphotriester intermediates
Author(s) -
J. E. Marugg,
Norbert Piel,
Larry W. McLaughlin,
M. Tromp,
G. H. Veeneman,
G.A. van der Marel,
Jacques H. van Boom
Publication year - 1984
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/12.22.8639
Subject(s) - dna , biology , phosphate , dna synthesis , phosphorylation , polymer , biochemistry , combinatorial chemistry , organic chemistry , chemistry
Phosphorylation of N-acyl-5'-O-DMTr-d-nucleosides, or similarly protected DNA-dimers having a free 3'-OH group, with 2-chlorophenyl-0,0-bis(1-benzotriazolyl)phosphate affords reactive 3'-phosphotriester derivatives. The latter intermediates can be used, without further purification, for the synthesis of DNA-fragments on the controlled pore glass/long chain alkylamine support. Further, 2-cyano-1,1-dimethylethoxy dichlorophosphine proved to be very suitable for the preparation of 5'-phosphorylated DNA-fragments on the same type of solid support.