Open AccessConformational changes induced in DNA by thein vitroreaction with the mutagenic amine: 3-N,N-acetoxyacetylainnio-4,6-dimethryldipyrido (l,2-a:3′,2′-d) imidazole
Author(s) -
Eric Hébert,
B. Loukakou,
G. SaintRuf,
Marc Leng
Publication year - 1984
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/12.22.8553
Subject(s) - circular dichroism , guanine , dna , in vitro , stereochemistry , biology , covalent bond , imidazole , amine gas treating , biochemistry , chemistry , nucleotide , organic chemistry , gene
The conformation of synthetic or natural DNAs modified in vitro by covalent binding of N-AcO-A-Glu-P-3 was investigated by fluorescence and circular dichroism. In all cases, substitution occurs mainly on the C8 of guanine residues. In modified poly(dG-dC).poly(dG-dC) or poly(dA-dC).poly(dG-dT) in B conformation, A-Glu-P-3 residues interact strongly with the bases whereas in Z conformation these residues are largely exposed to the solvent and interact weakly with the bases. A-Glu-P-3 and N-acetyl-2-aminofluorene (AAF) residues are equally efficient to induce the B-Z transition of poly(dG-dC).poly(dG-dC) and of poly(dA-dC).poly(dG-dT). Modifications of poly(dG).poly(dC) and calf thymus DNA indicate strong interactions between A-Glu-P-3 and the bases.