Open AccessOligonudeotides complementary to a promoter over the region −8… + 2 as transcription primers forE. coliRNA polymerase
Author(s) -
М.А. Грачев,
Evgeny Zaychikov,
Е. М. Иванова,
Н. И. Комарова,
Igor V. Kutyavin,
N.P. Sidelnikova,
Iana Frolova
Publication year - 1984
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/12.22.8509
Subject(s) - biology , ribonucleoside , oligonucleotide , primer (cosmetics) , microbiology and biotechnology , transcription (linguistics) , rna polymerase , deoxyribonucleotides , polymerase , nucleotide , rna , biochemistry , dna , gene , chemistry , linguistics , philosophy , organic chemistry
Primer-dependent transcription by E. coli RNA polymerase on T7 promoter A2 has been studied. Synthetic deoxyribonucleotides complementary to the promoter over the region -8...+2 were taken as primers. A ribonucleoside residue was present at the 3'-end of some of these oligonucleotides. The octanucleotide complementary to the region -8...-1 appeared to be an active primer. Oligonucleotides having lengths from 3 to 6 nucleotide residues complementary to the promoter over the region -4...+2 also exhibited primer activity. The latter was some 5-10 times greater in the case of oligonucleotides having a ribonucleoside residue at the 3'-end. Oligonucleotides which on complementary binding do not reach the center of phosphodiester bond synthesis, as well as the decanucleotides (-8...+2) and octanucleotides (-6...+2) of both the ribo- and deoxyribo-series were inactive as primers.