Open AccessKLONER; a computer program lo simulate recombinant DNA strategies by restriction map manipulation
Author(s) -
Paul L. Caron
Publication year - 1984
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/12.1part2.731
Subject(s) - restriction enzyme , biology , restriction map , restriction fragment , restriction digest , restriction site , dna , terminal restriction fragment length polymorphism , genetics , sticky and blunt ends , in vitro recombination , molecular cloning , recombinant dna , amplified fragment length polymorphism , ecori , computational biology , restriction fragment length polymorphism , gene , base sequence , polymerase chain reaction , complementary dna , population , demography , sociology , genetic diversity
A computer program is described which allows for the manipulation of restriction maps of various DNA fragments to demonstrate techniques used in DNA cloning and to predict and/or confirm experimental results. This program is capable of reading in restriction enzyme cleavage sites for several different DNA molecules of interest. This information is then compiled in order to form restriction maps which can then be processed by digestion with restriction endonucleases and treatment with other common DNA modifying enzymes. Ligation can then be simulated by joining fragments with complementary ends in all possible orientations, producing restriction maps of the products. The resulting recombinants can then be further analyzed by physical mapping with appropriate restriction endonucleases. This program was written in Pascal on an Apple II computer.