Open AccessCorrelation between suppressed meiotic recombination and the lack of DNA strand-breaks in the rRNA genes ofSaccharomyces cerevisiae
Author(s) -
Anders Høgset,
Tordis B. Øyen
Publication year - 1984
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/12.18.7199
Subject(s) - rad52 , biology , homologous recombination , genetics , meiosis , saccharomyces cerevisiae , ectopic recombination , recombination , genetic recombination , homologous chromosome , non allelic homologous recombination , flp frt recombination , gene , dna repair , dna , mlh1 , mutant , chromosome , rad51 , dna mismatch repair
We have examined whether the suppressed homologous meiotic recombination within the rDNA of S. cerevisiae is reflected by a lack of possibly recombination-initiating strand-breaks in this part of the genome. Our findings indicate that bulk DNA in the ds-break repair deficient mutant rad52/rad52 accumulates a limited number of both ss- and ds-breaks during meiosis as compared to a RAD+/rad52 heterozygote. The rDNA-containing chromosome is however protected against these breaks, and thus this may be an explanation for the suppression of recombination in the rDNA. The fact that ds-breaks seem to be involved gives indirect support to the ds-break-repair model for recombination.