Open AccessCodon usage can affect efficiency of translation of genes inEscherichia coli
Author(s) -
Martyn K. Robinson,
R.J. Lilley,
Stephen F. Little,
J.S. Emtage,
Geoffrey Yarranton,
Stephens Pe,
A. Millican,
Michael A. W. Eaton,
G. O. Humphreys
Publication year - 1984
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/12.17.6663
Subject(s) - division (mathematics) , library science , biology , computer science , mathematics , arithmetic
By inserting synthetic oligonucleotides into a highly expressed gene in E. coli it has been shown that unfavourable codon usage can reduce the maximum translation rate of a protein. However, in the case of the codon used (AGG), a significant effect on translation was only seen at very high transcription rates from a gene containing multiple copies of the unfavourable codon.