Open AccessThe histone HS gene is flanked by S1 hypersensitive structures
Author(s) -
Adolfo Ruiz-Carrilo
Publication year - 1984
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/12.16.6473
Subject(s) - biology , plasmid , dna , recombinant dna , gene , nuclease , microbiology and biotechnology , homology (biology) , dna supercoil , histone , hypersensitive site , genetics , deoxyribonuclease i , cleavage (geology) , restriction map , nucleosome , base sequence , dna replication , paleontology , fracture (geology)
The potential of the cloned histone H5 gene to form altered DNA structures has been examined by S1 nuclease digestion of supercoiled recombinant plasmids containing up to 8.8 kbp of chicken DNa. The three main nicking sites map at the upstream and downstream sequences flanking the structural gene. The cleavage sites share sequence homology, strand specificity, and do not seem to be single-stranded. The sequence of the S1-sensitive sites does not suggest that the fragments can adopt any of the known DNA secondary structures.