Open AccessDNA sequence and transcription of the region upstream of theE. coli gyrB gene
Author(s) -
Toshiro Adachi,
Kiyoshi Mizuuchi,
Rolf Menzel,
Martin Gellert
Publication year - 1984
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/12.16.6389
Subject(s) - biology , genetics , gene , transcription (linguistics) , dna , sequence (biology) , dna sequencing , escherichia coli , nucleic acid sequence , microbiology and biotechnology , linguistics , philosophy
We have determined the sequence of a 1498 base-pair region in E. coli that extends from within dnaN through recF and into the gyrB gene. An open reading frame of 1071 base pairs has been identified with the recF structural gene. By S1 mapping, we have located a transcription start point 31 base pairs upstream of gyrB. The amount of this transcript is much greater in cells that have been treated with novobiocin, a treatment which is known to induce greater synthesis of DNA gyrase.