Open AccessExpresion of therpsOandpnpgenes: structural analysis of a DNA fragment carrying their control regions
Author(s) -
Claude Portier,
Philippe Régnier
Publication year - 1984
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/12.15.6091
Subject(s) - biology , gene , polynucleotide phosphorylase , mutant , genetics , nucleic acid sequence , regulatory sequence , coding region , promoter , microbiology and biotechnology , gene expression , dna , ribosomal protein , purine nucleoside phosphorylase , rna , biochemistry , ribosome , enzyme , purine
Precise physical mapping of the genes rpsO and pnp coding respectively for ribosomal protein S15 and polynucleotide phosphorylase together with regions involved in the regulation of their expression has been obtained by the analysis of in vitro deletion mutants. The results suggest that each gene has its own promotor, but that there is coexpression of rpsO and pnp. The nucleotide sequence of rpsO and of the beginning of pnp is presented and includes the presumed regulatory regions of these genes. Several features of the sequence support the mapping experiments and are discussed in relation to the expression of the ribosomal and pnp genes.