Open AccessNucleotide sequence and transcription analysis of a linear DNA plasmid associated with the killer character of the yeastKhuyveromyces lactis
Author(s) -
Michael J. R. Stark,
Alan J. Mileham,
Michael A. Romanos,
Alan Boyd
Publication year - 1984
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/12.15.6011
Subject(s) - kluyveromyces lactis , biology , plasmid , open reading frame , gene , nucleic acid sequence , genetics , yeast , dna , kluyveromyces , structural gene , microbiology and biotechnology , saccharomyces cerevisiae , peptide sequence , escherichia coli
In killer strains of the yeast Kluyveromyces lactis, production of a protein toxin which inhibits the growth of sensitive yeast cells is associated with the presence of two linear DNA plasmids, k1 and k2. We have determined the nucleotide sequence of the smaller plasmid k1 (8.9kb) which is thought to carry the structural gene(s) encoding the toxin. The plasmid has a low G + C content (26.8%) and contains four long open reading frames which account for over 95% of the total sequence. The longest open reading frame (1146 amino acids) probably corresponds to a structural gene for the killer toxin. Transcripts from three of the putative genes have been detected in K.lactis by Northern hybridisation.