Open AccessA type II restriction endonuclease with an eight nucleotide specificity fromStreptomyces fimbriatus
Author(s) -
Boqin Qiang,
Ira Schildkraut
Publication year - 1984
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/12.11.4507
Subject(s) - biology , restriction enzyme , endonuclease , nucleotide , genetics , microbiology and biotechnology , recognition sequence , nucleic acid sequence , streptomycetaceae , dna , streptomyces , actinomycetales , gene , bacteria
A new site-specific endonuclease, Sfi I, has been isolated from Streptomyces fimbriatus . This is the first report of a type II restriction endonuclease whose recognition specificity requires eight nucleotides. Sfi I cleaves the sequence, GGCCNNNN / NGGCC , symmetrically to produce a three base, 3' extension.