A type II restriction endonuclease with an eight nucleotide specificity fromStreptomyces fimbriatus | Zendy

Boqin Qiang | Zendy; Ira Schildkraut | Zendy

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A type II restriction endonuclease with an eight nucleotide specificity fromStreptomyces fimbriatus

Author(s) -

Boqin Qiang,

Ira Schildkraut

Publication year - 1984

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/12.11.4507

Subject(s) - biology , restriction enzyme , endonuclease , nucleotide , genetics , microbiology and biotechnology , recognition sequence , nucleic acid sequence , streptomycetaceae , dna , streptomyces , actinomycetales , gene , bacteria

A new site-specific endonuclease, Sfi I, has been isolated from Streptomyces fimbriatus . This is the first report of a type II restriction endonuclease whose recognition specificity requires eight nucleotides. Sfi I cleaves the sequence, GGCCNNNN / NGGCC , symmetrically to produce a three base, 3' extension.

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