Open AccessThe 245 base-pairoriCsequence of theE. colichromosome directs bidirectional replication at an adjacent region
Author(s) -
Satoshi Tabata,
Atsuhiro Oka,
Keiki Sugimoto,
Mituru Takanami,
Soichi Yasuda,
Y Hirota
Publication year - 1983
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/11.9.2617
Subject(s) - biology , genetics , sequence (biology) , origin of replication , base pair , chromosome , base sequence , dna replication , base (topology) , dna , replication (statistics) , microbiology and biotechnology , gene , virology , mathematical analysis , mathematics
The replication origin of the E. coli K-12 chromosome has been isolated as autonomously replicating molecules(oriC plasmid), and the DNA region essential for replicating function(oriC) has been localized to a sequence of 232-245 base-pairs(bp) by deletion analysis. In this report, the functional role of oriC was analysed by using an in vitro replication system and various OriC+ and OriC- plasmids previously constructed. The results obtained were summarized as follows: (1) The oriC sequence contained information enough to direct bidirectional replication. (2) The actual DNA replication began at a region near, but outside, oriC and progressed bidirectionally. (3) Initiation of DNA synthesis at the specific region required the dnaA-complementing fraction from cells harboring a dnaA-carrying plasmid.