Open AccessExpression inSaccharomyces cerevisiaeof human interferon-alpha directed by theTRP1 5′region
Author(s) -
Melanie J. Dobson,
Mick F. Tuite,
Jane Mellor,
Nigel Roberts,
R King,
D. C. Burke,
Alan J. Kingsman,
Susan M. Kingsman
Publication year - 1983
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/11.8.2287
Subject(s) - biology , saccharomyces cerevisiae , coding region , gene , genetics , nucleic acid sequence , complementary dna , peptide sequence , tata box , transcription (linguistics) , upstream activating sequence , microbiology and biotechnology , gene expression , promoter , linguistics , philosophy
The complete 5' flanking region of the yeast TRP1 gene encoding N-(5'-phosphoribosyl)- anthranilate isomerase, a nonabundant protein, has been cloned and the nucleotide sequence data has been extended from -102 to -440. The CT block--CAAG structure common to all efficiently expressed yeast genes is altered in the 5' region of TRP1 and a sequence postulated to be involved in general amino acid regulation is absent. There are two possible TATA boxes at -224 and -262. TRP1, in common with HIS3, HIS4 and TRP5 has a region of dyad symmetry upstream of the coding sequence which may play a role in initiation of transcription. The relative efficiency of gene expression directed by the complete 5' TRP1 region was assessed by comparison with that of PGK by inserting a cDNA for a human interferon-alpha downstream of their respective 5' regions. The respective interferon yields indicate that their in vivo expression capabilities are a function of their 5' regions.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom