Open AccessExpression inSaccharomyces cerevisiaeof human interferon-alpha directed by theTRP1 5′region
Author(s) -
Melanie J. Dobson,
Mick F. Tuite,
Jane Mellor,
Nigel Roberts,
R King,
D. C. Burke,
Alan J. Kingsman,
Susan M. Kingsman
Publication year - 1983
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/11.8.2287
Subject(s) - biology , saccharomyces cerevisiae , coding region , gene , genetics , nucleic acid sequence , complementary dna , peptide sequence , tata box , transcription (linguistics) , upstream activating sequence , microbiology and biotechnology , gene expression , promoter , linguistics , philosophy
The complete 5' flanking region of the yeast TRP1 gene encoding N-(5'-phosphoribosyl)- anthranilate isomerase, a nonabundant protein, has been cloned and the nucleotide sequence data has been extended from -102 to -440. The CT block--CAAG structure common to all efficiently expressed yeast genes is altered in the 5' region of TRP1 and a sequence postulated to be involved in general amino acid regulation is absent. There are two possible TATA boxes at -224 and -262. TRP1, in common with HIS3, HIS4 and TRP5 has a region of dyad symmetry upstream of the coding sequence which may play a role in initiation of transcription. The relative efficiency of gene expression directed by the complete 5' TRP1 region was assessed by comparison with that of PGK by inserting a cDNA for a human interferon-alpha downstream of their respective 5' regions. The respective interferon yields indicate that their in vivo expression capabilities are a function of their 5' regions.