Open AccessSimultaneous analysis of conformation and transcription of A and B groups of vitellogenin genes in male and femaleXenopusdaring primary and secondary activation by estrogen
Author(s) -
J. L. Williams,
Jamshed R. Tata
Publication year - 1983
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/11.4.1151
Subject(s) - vitellogenin , biology , xenopus , gene , estrogen , transcription (linguistics) , stimulation , transcription factor , medicine , microbiology and biotechnology , endocrinology , genetics , linguistics , philosophy
In male Xenopus, primary estradiol administration results in noncoordinate activation in the liver of the A and B groups of vitellogenin genes, both as judged by transcription and DNase I sensitivity in isolated nuclei, B group genes being activated preferentially in the first 20 hr. Secondary induction in males or "primary" induction in females results in a coordinate and equal transcription of these two groups of genes. The elevated transcriptional activity following primary estrogen stimulation returns to low levels rapidly but the high DNase I sensitivity of these genes persists for 2-3 months. A non-coordinate activation of the A and B groups of vitellogenin genes is however re-established in response to a second administration of estradiol 8 months after primary stimulation of male Xenopus.