Open AccessThe nucteotide sequence of myodn light chain (L-2A) mRNA from embryonic chicken cardiac muscle tissue
Author(s) -
Hans Henning Arnold,
Manuel Krauskopf,
M.A.Q. Siddiqui
Publication year - 1983
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/11.4.1123
Subject(s) - biology , messenger rna , cardiac muscle , immunoglobulin light chain , embryo , embryonic stem cell , embryogenesis , microbiology and biotechnology , myocyte , sequence (biology) , anatomy , genetics , gene , antibody
The nucleotide sequence of a cDNA clone (pML10) for chicken cardiac myosin light chain is described. The cDNA insert contains 613 nucleotides representing the entire coding sequence, with the exception of nine NH2-terminal amino acids, and the full 3'-non-coding region of 146 nucleotides. The missing 5' terminus of the mRNA, not represented in the clone pML10, was obtained by extension of the cDNA using a 43 nucleotide long internal EcoR1 fragment as a primer. The non-coding region contains several direct and inverted repeated sequences and the polyadenylation signal sequence AATAAA. The coding portion exhibits non-random usage of synonymous codons with a strong bias for codons ending in G and C.