The nucteotide sequence of myodn light chain (L-2A) mRNA from embryonic chicken cardiac muscle tissue | Zendy

Hans-Henning Arnold | Zendy; M. Krauskopf | Zendy; M.A.Q. Siddiqui | Zendy

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The nucteotide sequence of myodn light chain (L-2A) mRNA from embryonic chicken cardiac muscle tissue

Author(s) -

Hans-Henning Arnold,

M. Krauskopf,

M.A.Q. Siddiqui

Publication year - 1983

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/11.4.1123

Subject(s) - biology , messenger rna , cardiac muscle , immunoglobulin light chain , embryo , embryonic stem cell , embryogenesis , microbiology and biotechnology , myocyte , sequence (biology) , anatomy , genetics , gene , antibody

The nucleotide sequence of a cDNA clone (pML10) for chicken cardiac myosin light chain is described. The cDNA insert contains 613 nucleotides representing the entire coding sequence, with the exception of nine NH2-terminal amino acids, and the full 3'-non-coding region of 146 nucleotides. The missing 5' terminus of the mRNA, not represented in the clone pML10, was obtained by extension of the cDNA using a 43 nucleotide long internal EcoR1 fragment as a primer. The non-coding region contains several direct and inverted repeated sequences and the polyadenylation signal sequence AATAAA. The coding portion exhibits non-random usage of synonymous codons with a strong bias for codons ending in G and C.

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