Sequence of the nucleocapsid gene from murine coronavirus MHV-A59 | Zendy

J. Scott Armstrong | Zendy; Sjef Smeekens | Zendy; Peter J. M. Rottier | Zendy

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Sequence of the nucleocapsid gene from murine coronavirus MHV-A59

Author(s) -

J. Scott Armstrong,

Sjef Smeekens,

Peter J. M. Rottier

Publication year - 1983

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/11.3.883

Subject(s) - biology , nucleic acid sequence , microbiology and biotechnology , open reading frame , coronavirus , gene , virology , sequence (biology) , peptide sequence , dna , rna , genetics , covid-19 , medicine , disease , pathology , infectious disease (medical specialty)

The nucleotide sequence of the RNA encoding the nucleocapsid protein of coronavirus MHV-A59 has been determined. Copy DNA was prepared from mRNA isolated from virally infected cells, fragmented and cloned in the phage vector M13 mp8 for direct sequence determination. A sequence of 1817 nucleotides, adjacent to the viral poly-A tail, was obtained. It contains a single long open reading frame encoding a protein of mol. wt. 49660, which is enriched in basic residues.

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