Open AccessNucleotide sequence of theEsherichia colixanthine-guanine phosphoribosyl transferase gene
Author(s) -
Dickson Pratt,
Suresh Subramani
Publication year - 1983
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/11.24.8817
Subject(s) - biology , guanine , nucleic acid sequence , hypoxanthine , gene , biochemistry , microbiology and biotechnology , transferase , nucleotide , peptide sequence , coding region , phosphoribosyltransferase , hypoxanthine guanine phosphoribosyltransferase , xanthine , genetics , enzyme , mutant
The Escherichia coli gene coding for the enzyme xanthine-guanine phosphoribosyl transferase (gpt) has been widely used as a dominant selectable marker in a variety of mammalian cells. We have determined the complete nucleotide sequence of the 1057 base pair (bp) segment of DNA containing this gene. The coding sequence for the enzyme is 456 nucleotides long and can code for a 152 amino acid (16.9 Kd) polypeptide. A comparison of the amino acid sequence of the bacterial enzyme with that of the mammalian hypoxanthine-guanine phosphoribosyl transferase (hprt) reveals no significant homology between the two polypeptides.