Open AccessSixSchizosaccharomyces pombetRNA genes including a gene for a tRNALyswith an intervening sequence which cannot base-pair with the anticodon
Author(s) -
Vera Gamulin,
Jen-i Mao,
Bernd Appel,
Martin Sumner-Smith,
Fumiaki Yamao,
Dieter Söll
Publication year - 1983
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/11.24.8537
Subject(s) - haven , transfer rna , biology , computational biology , biochemistry , library science , gene , rna , computer science , mathematics , combinatorics
We report the sequences of six S. pombe tRNA genes including two genes for tRNAArg, and one gene each for tRNAGlu, tRNAHis, tRNALys and tRNAPhe. All tRNA genes are found independently in the genome and represent individual transcription units. The gene for tRNALys has an 8 bp long intervening sequence which cannot base-pair with the tRNA anticodon. In vitro transcription studies indicate that all genes are faithfully transcribed in a yeast extract. Sequence comparison of the 5' flanking regions of the tRNA genes did not show significant homologies; however, they are very rich in AT base pairs.