Open AccessTwo nuclear DNA binding proteins ofDictyostelium discoideumwith a high affinity for poly(dA)-poly(dT)
Author(s) -
Hervé Garreau,
Jason Williams
Publication year - 1983
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/11.23.8473
Subject(s) - dictyostelium discoideum , biology , intergenic region , dictyostelium , gene , nucleic acid sequence , dna , genome , dna binding protein , genetics , microbiology and biotechnology , nuclear dna , transcription factor , mitochondrial dna
Intergenic regions of the Dictyostelium genome contain an extremely high proportion of AT base pairs. Those intergenic regions which have been subjected to nucleotide sequence analysis are predominantly composed of alternating runs of poly(dA) and poly(dT) and there is evidence to suggest that nucleosomes do not form on such sequences. We have identified two nuclear proteins, of molecular weight 70,000 and 74,000 daltons, which bind only to intergenic regions of a cloned Dictyostelium gene. Binding is specifically inhibited in the presence of synthetic poly(dA) - poly (dT) as competitor. These proteins may play some role in the chromosomal organization of intergenic regions in Dictyostelium discoideum.