Mas spectrometric monitoring of solid phase phosphate triester synthesis of DNA fragments | Zendy

Lutz Alder | Zendy; André Rosenthal | Zendy; Dieter Cech | Zendy

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Mas spectrometric monitoring of solid phase phosphate triester synthesis of DNA fragments

Author(s) -

Lutz Alder,

André Rosenthal,

Dieter Cech

Publication year - 1983

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/11.23.8431

Subject(s) - oligonucleotide , pyrolysis , phosphate , mass spectrometry , polymer , dna , base (topology) , solid phase synthesis , chromatography , biology , phase (matter) , combinatorial chemistry , analytical chemistry (journal) , biochemistry , organic chemistry , chemistry , mathematical analysis , peptide , mathematics

A new and convenient pyrolysis mass spectrometric approach for monitoring solid phase phosphate triester synthesis of oligodeoxyribonucleotides in 5'-3' direction has been developed. The base-typical fragment ions produced by pyrolysis at 280 degrees C of the polymer-bound oligonucleotide triesters in the mass spectrometer permit the analytical monitoring of DNA chain growth, using simple mathematical operations. The base composition can be determined directly from the polymer. In addition, minor nucleosides can easily be detected.

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