Open AccessNucleotide sequence and stability of the RNA component of RNase P from a temperature-sensitive mutant ofE. coli
Author(s) -
Hiroshi Sakamoto,
Naohiro Kimura,
Fumikiyo Nagawa,
Yoichi Shimura
Publication year - 1983
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/11.23.8237
Subject(s) - biology , rnase p , rna , mutant , rnase h , microbiology and biotechnology , nucleic acid sequence , non coding rna , gene , nuclease protection assay , rnase mrp , nucleotide , ribonuclease iii , dna , coding region , genetics , rna interference
The gene coding for the RNA component of RNase P was cloned from a temperature-sensitive mutant of Escherichia coli defective in RNase P activity (ts709) and its parental wild-type strain (4273), and the complete nucleotide sequences of the gene and its flanking regions were determined. The 5'- and 3'-terminal sequences of the RNA component were determined and mapped on the DNA sequence. The mutant gene has GC-to-AT substitutions at positions corresponding to 89 and 365 nucleotides downstream from the 5' terminus of the RNA sequence. Comparing to the wild-type RNA, the mutant RNA is less stable and rapidly degraded in vivo and in vitro.