Open AccessT4 DNA ligase can seal a nick in double-stranded DNA limited by a 5′-phosphoryiated base-free deoxyribose residue
Author(s) -
Colette Goffin,
Walter G. Verly
Publication year - 1983
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/11.22.8103
Subject(s) - phosphodiester bond , deoxyribose , dna ligase , ap site , biology , dna , dna ligases , sticky and blunt ends , biochemistry , microbiology and biotechnology , endonuclease , rna , gene
The 5' AP endodeoxyribonucleases hydrolyze the phosphodiester bond 5' to AP (apurinic or apyrimidinic) sites in double-stranded DNA leaving 3'-OH and 5'-phosphate ends. These nicks are sealed by T4 DNA ligase although the 5'-phosphate end belongs to a base-free deoxyribose.