Open AccessNucleotide sequence of the 5′ noncoding region and part of thegaggene of mouse mammary tumor virus; identification of the 5′ splicing site for subgenomic mRNAs
Author(s) -
Nicolás Fasel,
Elena Buetti,
J M Firzlaff,
Karen W. Pearson,
Heidi Diggelmann
Publication year - 1983
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/11.20.6943
Subject(s) - biology , subgenomic mrna , gene , rna splicing , genetics , mouse mammary tumor virus , nucleic acid sequence , virus , sequence (biology) , microbiology and biotechnology , virology , rna
We have determined the sequence of the first 1371 nucleotides at the 5' end of the genome of mouse mammary tumor virus using molecularly cloned proviral DNA of the GR virus strain. The most likely initiation codon used for the gag gene of mouse mammary tumor virus is the first one, located 312 nucleotides from the 5' end of the viral RNA. The 5' splicing site for the subgenomic mRNA's is located approximately 288 nucleotides downstream from the 5' end of the viral RNA. From the DNA sequence the amino acid sequence of the N-terminal half of the gag precursor protein, including p10 and p21, was deduced (353 amino acids).