Open AccessNucleotide sequence of the amylase gene fromBacillus subtilis
Author(s) -
Maria Yang,
Alessandro Galizzi,
Dennis J. Henner
Publication year - 1983
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/11.2.237
Subject(s) - biology , bacillus subtilis , gene , nucleic acid sequence , genetics , coding region , open reading frame , pbr322 , escherichia coli , peptide sequence , molecular cloning , microbiology and biotechnology , bacteria
The gene coding for amylase (EC.3.2.1.1) has been isolated and sequenced from Bacillus subtilis by cloning in lambda Charon4A and pBR322. The entire coding sequence and large preceding and following regions, comprising the presumed transcriptional and translational regulatory regions, were sequenced. The coding sequence shows a large open reading frame with a translated molecular weight of 72,800 and a presumed signal sequence of approximately thirty-two amino acids. When the intact gene is present in Escherichia coli, it confers the ability to degrade starch, indicating that the gene is expressed in a functional state.