Essential structure ofE. colipromoter effect of spacer length between the two consensus sequences on promoter function | Zendy

Takashi Aoyama | Zendy; Mituru Takanami | Zendy; Eiko Ohtsuka | Zendy; Yoshio Taniyama | Zendy; Ryuji Marumoto | Zendy; Hiroko Sato | Zendy; M Ikehara | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Essential structure ofE. colipromoter effect of spacer length between the two consensus sequences on promoter function

Author(s) -

Takashi Aoyama,

Mituru Takanami,

Eiko Ohtsuka,

Yoshio Taniyama,

Ryuji Marumoto,

Hiroko Sato,

M Ikehara

Publication year - 1983

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/11.17.5855

Subject(s) - biology , consensus sequence , transcription (linguistics) , genetics , promoter , sequence (biology) , transcription factor , function (biology) , dna , base sequence , gene , gene expression , linguistics , philosophy

A promoter with the consensus sequence(TTGACA and TATAAT) at -35 and -10 regions was constructed, and the distance between the two consensus sequences was independently altered at two restriction sites. In an in vitro transcription system, a maximum activity was observed at the spacer length of 17 base-pairs regardless of the sites of space adjustment. Evidence was also provided that the start point of transcription was fixed by the consensus sequence at the -10 region.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research