Open AccessEssential structure ofE. colipromoter effect of spacer length between the two consensus sequences on promoter function
Author(s) -
Takashi Aoyama,
Mituru Takanami,
Eiko Ohtsuka,
Yoshio Taniyama,
Ryuji Marumoto,
Hiroko Sato,
M. Ikehara
Publication year - 1983
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/11.17.5855
Subject(s) - biology , consensus sequence , transcription (linguistics) , genetics , promoter , sequence (biology) , transcription factor , function (biology) , dna , base sequence , gene , gene expression , linguistics , philosophy
A promoter with the consensus sequence(TTGACA and TATAAT) at -35 and -10 regions was constructed, and the distance between the two consensus sequences was independently altered at two restriction sites. In an in vitro transcription system, a maximum activity was observed at the spacer length of 17 base-pairs regardless of the sites of space adjustment. Evidence was also provided that the start point of transcription was fixed by the consensus sequence at the -10 region.