Open AccessIn vivoregulation of theuvrAgene: role of the “−10” and “−35” promoter regions
Author(s) -
Claude Backendorf,
Jourica A. Brandsma,
Tonja Kartašova,
Pieter van de Putte
Publication year - 1983
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/11.17.5795
Subject(s) - biology , repressor lexa , transcription (linguistics) , microbiology and biotechnology , gene , genetics , promoter , plasmid , transcription factor , gene expression , repressor , linguistics , philosophy
The effect of increasing deletions in the uvrA promoter region on the transcriptional efficiency was quantitatively analysed by fusion to the galK structural gene. A physical analysis of uvrA messenger RNA synthesis from the different deletion plasmids was performed using the S1 mapping technique. Both methods indicate that the uvrA "-10" promoter sequence is sufficient to trigger uvrA transcription. Although not essential, the "-35" region, which is overlapping with the LexA binding site, is shown to have an enhancing function, as the exposure of this region after SOS induction results in a 3- to 4-fold increase in uvrA transcription. A model is presented which accounts both for the observed basal and induced expression of the uvrA gene on a molecular level.