Open AccessComplete nucleotide sequence of theEscherichia coli gdhAgene
Author(s) -
Michael J. McPherson,
John C. Wootton
Publication year - 1983
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/11.15.5257
Subject(s) - biology , gene , genetics , nucleic acid sequence , escherichia coli , peptide sequence , inverted repeat , neurospora , microbiology and biotechnology , neurospora crassa , genome , mutant
The DNA sequence of the gdhA gene of Escherichia coli K12, which encodes the 447 amino acid polypeptide subunit of NADP-specific glutamate dehydrogenase, is presented. The deduced protein sequence is strongly homologous to the corresponding enzyme of the eukaryotic fungus Neurospora crassa. The upstream DNA sequence includes several overlapping promoter consensus sequences. The downstream DNA sequence contains inverted repeats, predicted as forming long stable stem-loop structures in RNA, homologous to those found in several enterobacterial intergenic regions.