Open AccessDifferent levels of induction ofRecAprotein inE. coli(PQ 10) after treatment with two related carcinogens
Author(s) -
Bernard Salles,
Marie Claude E. Lang,
Anne-Marie Freund,
Claude Paoletti,
Michel Daune,
Robert Fuchs
Publication year - 1983
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/11.15.5235
Subject(s) - 2 acetylaminofluorene , biology , carcinogen , adduct , dna , escherichia coli , sos response , microbiology and biotechnology , immunoradiometric assay , biochemistry , genetics , dna repair , gene , enzyme , chemistry , radioimmunoassay , organic chemistry , microsome
By means of an immunoradiometric assay the induction of protein RecA in E. coli PQ 10 was measured after treatment by two related carcinogens. On an adduct basis N-Acetoxy-N-2-acetylaminofluorene was shown to induce the protein RecA at a similar level as U.V. On the other hand, N-hydroxy-N-2-aminofluorene shows only a poor induction capacity of the RecA protein. The difference in the SOS inducing potential of the aminofluorene and acetylaminofluorene adducts is discussed in relation to the major difference in the local conformational change the two adducts induce in DNA.