Open AccessA repetitive and apparently transposable DNA sequence inDictyostelium discoideumassociated with developmentally regulated RNAs
Author(s) -
Sungwon Chung,
Charles S. Zuker,
Harvey F. Lodish
Publication year - 1983
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/11.14.4835
Subject(s) - biology , transposable element , dictyostelium discoideum , dictyostelium , genetics , genome , repeated sequence , conserved sequence , dna , dna sequencing , sequence analysis , restriction fragment , restriction map , genomic dna , nucleic acid sequence , gene , base sequence
Dictyostelium discoideum AX3 genomic DNA contains about 40 copies of a 4.5 Kb sequence. Each has the same restriction map, suggesting that they are all very similar. The sequences are scattered throughout the genome, and each of the six representative copies we cloned have different flanking sequences. Partial fragments of the 4.5 Kb sequence also are scattered throughout the genome. The DNA sequences flanking the 4.5 Kb repetitive sequences are different in different strains of Dictyostelium suggesting that the 4.5 Kb sequence is a transposable element. Each sub-region of this 4.5 Kb sequence is associated with a large number of mRNAs, all of which are developmentally regulated, but whose function is not known.