Open AccessBlocked 5′-termini in the fragments of chromosomal DNA produced in cells exposed to the antitumour drug 4′-[(9-acridinyl)-amino]methanesulphon-m-anisidide (mAMSA)
Author(s) -
Brendan Marshall,
R.K. Ralph,
Ronald Hancock
Publication year - 1983
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/11.12.4251
Subject(s) - biology , dna , microbiology and biotechnology , amsacrine , genetics , topoisomerase
Comparison of the sensitivity of DNA isolated from untreated and mAMSA-treated PY815 mouse mastocytoma cells to hydrolysis by E.coli 3'-exonuclease III and phage lambda or phage T7 5'-exonucleases show that the fragments of chromosomal DNA produced by mAMSA treatment have free 3'-OH termini and blocked 5'-termini.