Open AccessA1H nOe and CD study of the salt-concentration dependence of the structure of d(G-C)5
Author(s) -
David G. Reid,
Stephen A. Salisbury,
Dudley H. Williams
Publication year - 1983
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/11.11.3779
Subject(s) - duplex (building) , nuclear overhauser effect , sodium , salt (chemistry) , nuclear magnetic resonance spectroscopy , spectral line , crystallography , biology , nuclear magnetic resonance , dna , chemistry , biochemistry , physics , organic chemistry , astronomy
Intra- and internucleotide 1H nuclear Overhauser effects (nOes) have been observed in the synthetic decancleoside nonaphosphate d(G-C)5 at low and high salt concentrations, and in conditions under which duplex formation is complete. Comparison of the nOes with interproton distances derived from model and X-ray structures suggest that the duplex is right-handed up to a sodium chloride concentration of 4M, in contrast to the behaviour of poly [d(G-C)]. C.D. spectra are consistent with this suggestion.