Open AccessThe pR UV+plasmid, transfecled into mammalian cells, enhances their UV survival
Author(s) -
R. Elli,
L. Marcucci,
R Bosi,
C. Oppi,
Piero A. Battaglia,
F. Gigliani
Publication year - 1983
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/11.11.3679
Subject(s) - plasmid , biology , microbiology and biotechnology , dna , southern blot , genetics
It has been recently reported that the pR plasmid enhances the UV survival in E.coli c600. In order to test whether this function may be expressed also in mammalian cells, LTA (tk- aprt-) mouse cells were cotransformed with pR plasmid DNA and ptk1 plasmid as selectable marker. Tk+ transformants were analyzed for their UV survival and for the presence of pR DNA sequences by blot-hybridization. The results show a correlation between the enhanced UV survival and presence of pR DNA sequences in cotransformed LTA mouse cells.