Open AccessStructure of mottse rRNA precursors.Complete sequence and potential folding of the spacer regions between 18S and 28S rRNA
Author(s) -
Bernard Michot,
JeanPierre Bachellerie,
Françoise Raynal
Publication year - 1983
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/11.10.3375
Subject(s) - biology , ribosomal rna , internal transcribed spacer , ribosome , 18s ribosomal rna , genetics , 23s ribosomal rna , 28s ribosomal rna , homology (biology) , 5.8s ribosomal rna , rna , 5s ribosomal rna , spacer dna , nucleic acid sequence , gene , nucleotide , microbiology and biotechnology
We have determined the complete nucleotide sequence of the regions of mouse ribosomal RNA transcription unit which separate mature rRNA genes. These internal transcribed spacers (ITS) are excised from rRNA precursor during ribosome biosynthesis. ITS 1, between 18S and 5.8S rRNA genes, is 999 nucleotides long. ITS 2, between 5.8S and 28S rRNA genes, is 1089 nucleotides long. Both spacers are very rich in G + C, 70 and 74% respectively. Mouse sequences have been compared with the other available eukaryotes: while no homology is apparent with yeast or xenopus, mouse and rat ITS sequences have been largely conserved, with homologous segments interspersed with highly divergent tracts. Homology with rat is much more extensive for ITS 1 than for ITS 2. Tentative secondary structure models are proposed for the folding of these regions within rRNA precursor; they are closely related in mouse and rat.