Open AccessExpression of human immunoglobulin E ε chain cDNA inE.coli
Author(s) -
Tsutornu Kurokawa,
Masaharu Seno,
Reiko Sasada,
Yoshitaka Ona,
Haruo Onda,
Koichi Igarashi,
Masakazu Kikuchi,
Yoshio Sugino,
Tasuku Honjo
Publication year - 1983
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/11.10.3077
Subject(s) - antigenicity , biology , complementary dna , plasmid , microbiology and biotechnology , molecular cloning , peptide sequence , chain (unit) , j chain , molecular mass , antibody , gene , biochemistry , genetics , enzyme , physics , astronomy
Using the cDNA of human epsilon chain, three expression plasmids that code directly the constant portion of the epsilon chain (C epsilon 1-C epsilon 4, C epsilon 2-C epsilon 4 and C epsilon 3-C epsilon 4 domains) were constructed. These epsilon chain peptides were synthesized in E. coli under the control of the trp promoter-operator. The bacterially produced peptides have the antigenicity of human epsilon chain and gave the molecular weights equal to the values calculated from the amino acid sequence of the constructed plasmids.