Expression of human immunoglobulin E ε chain cDNA inE.coli | Zendy

Tsutornu Kurokawa | Zendy; Masaharu Seno | Zendy; Reiko Sasada | Zendy; Yoshitaka Ona | Zendy; Haruo Onda | Zendy; Koichi Igarashi | Zendy; Masakazu Kikuchi | Zendy; Yukio Sugino | Zendy; Tasuku Honjo | Zendy

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Expression of human immunoglobulin E ε chain cDNA inE.coli

Author(s) -

Tsutornu Kurokawa,

Masaharu Seno,

Reiko Sasada,

Yoshitaka Ona,

Haruo Onda,

Koichi Igarashi,

Masakazu Kikuchi,

Yukio Sugino,

Tasuku Honjo

Publication year - 1983

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/11.10.3077

Subject(s) - antigenicity , biology , complementary dna , plasmid , microbiology and biotechnology , molecular cloning , peptide sequence , chain (unit) , j chain , molecular mass , antibody , gene , biochemistry , genetics , enzyme , physics , astronomy

Using the cDNA of human epsilon chain, three expression plasmids that code directly the constant portion of the epsilon chain (C epsilon 1-C epsilon 4, C epsilon 2-C epsilon 4 and C epsilon 3-C epsilon 4 domains) were constructed. These epsilon chain peptides were synthesized in E. coli under the control of the trp promoter-operator. The bacterially produced peptides have the antigenicity of human epsilon chain and gave the molecular weights equal to the values calculated from the amino acid sequence of the constructed plasmids.

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