Studies on deoxyribonucleic acids and related compounds. V. Synthesis of pentadecanucleotide duplex containing the ideal Pribnow sequence of promoter by the phosphotriester method using a new phosphorylating reagent | Zendy

Eiko Ohtsuka | Zendy; Yoshio Taniyama | Zendy; Ryuji Marumoto | Zendy; Hiroko Sato | Zendy; Haruhisa Hirosaki | Zendy; Morio Ikehara | Zendy

Open Access

Studies on deoxyribonucleic acids and related compounds. V. Synthesis of pentadecanucleotide duplex containing the ideal Pribnow sequence of promoter by the phosphotriester method using a new phosphorylating reagent

Author(s) -

Eiko Ohtsuka,

Yoshio Taniyama,

Ryuji Marumoto,

Hiroko Sato,

Haruhisa Hirosaki,

Morio Ikehara

Publication year - 1982

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/10.8.2597

Subject(s) - oligonucleotide , reagent , duplex (building) , biology , sequence (biology) , nucleotide , phosphorylation , oligonucleotide synthesis , dna , combinatorial chemistry , ideal (ethics) , nucleic acid sequence , biochemistry , stereochemistry , organic chemistry , chemistry , gene , philosophy , epistemology

A phosphorylating reagent o-chlorophenyl phosphoro-p-anisi-dochloridate was synthesized to phosphorylate the 3'-hydroxyl group of N, 5'-protected deoxynucleosides. These nucleotides served as 3'-terminal units for the synthesis of oligonucleotide blocks. By condensation of these oligonucleotide blocks the partially complementary deoxypentadecanucleotides dAGCTTATAATGC-TCG and dAGCTCGAGCATTATA, which contained the ideal Pribnow sequence TATAATG, were synthesized.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research