Open AccessPrimary and secondary structure of S.8S rRNA from the silkgbnd ofBombyx mori
Author(s) -
Haruhiko Fujiwara,
Yukihiro Kawata,
Hajime Ishikawa
Publication year - 1982
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/10.7.2415
Subject(s) - bombyx mori , biology , ribosomal rna , protein primary structure , protein secondary structure , nucleic acid sequence , sequence (biology) , nucleotide , genetics , biochemistry , peptide sequence , gene
Nucleotide sequence of 5.8S rRNA of the silkworm, Bombyx mori has been determined by gel sequencing methods. The 5.8S rRNA was the longest so far reported, with the 5'-terminal sequence several nucleotides longer than those of the other organisms. Upon constructing the secondary structure in accordance with the "burp gun" model (12), the Bombyx 5.8S rRNA formed a wide-open "muzzle" due to several unpaired bases at the ends. The overall structure also appeared less stable with less G . C pairs and more unpaired bases than that of the HeLa 5.8S rRNA. These structural features may be essential for those 5.8S rRNAs which interact with 28S rRNAs containing the hidden break to form a stable complex.