Nucleosome spacing in rat liver cluomatin. A study with exonuclease III | Zendy

François Strauss | Zendy; Ariel Prunell | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Nucleosome spacing in rat liver cluomatin. A study with exonuclease III

Author(s) -

François Strauss,

Ariel Prunell

Publication year - 1982

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/10.7.2275

Subject(s) - nucleosome , micrococcal nuclease , biology , exonuclease iii , chromatin , base pair , dna , biophysics , nuclease , genetics , gene , escherichia coli

Exonuclease III was used to uniformly trim DNA ends of micrococcal nuclease-prepared chromatin fragments down to the first major impediment encountered by the enzyme, which arises from the interaction of H1 with the nucleosome. This trimming, when performed on nucleosome dimers, allowed one to quantitatively determine the center-to-center distance of nucleosomes. This distance, of mean 198 base pairs, was found to essentially vary between about 180 and 215 base pairs, with extremes of 165 and 230 base pairs. Trimming of trimers further revealed that the overall arrangement of nucleosome center-to-center distances along the chromatin fiber is that expected on a statistical basis.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research