Open AccessReplication of λ dv DNA in vitro
Author(s) -
Alfons Anderl,
Albrecht Klein
Publication year - 1982
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/10.5.1733
Subject(s) - biology , dna replication , plasmid , dna , ribonucleotide , dna gyrase , semiconservative replication , microbiology and biotechnology , in vitro , gene , dna synthesis , control of chromosome duplication , genetics , escherichia coli , nucleotide
Exogenous lambda dv DNA was replicated in extracts prepared from E. coli cells carrying plasmids with inducible lambda O and /or P genes. Extracts from cells carrying only one of the two lambda replication functions complement each other in this reaction. The reaction further requires ribonucleotide triphosphates, an ATP regenerating system, DNA gyrase and RNA polymerase functions. Density labelling of the superhelical reaction products results in hybrid density indicating that one complete round of replication has taken place in vitro.