Open AccessCloning and sequence of the crp gene of Escherichia coli K 12
Author(s) -
Pascale Cossart,
Brigitte Gicquel-Sanzey
Publication year - 1982
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/10.4.1363
Subject(s) - biology , nucleic acid sequence , pbr322 , escherichia coli , peptide sequence , gene , microbiology and biotechnology , cloning (programming) , genetics , molecular cloning , sequence (biology) , sequence analysis , computer science , programming language
We have determined the nucleotide sequence of the crp gene of Escherichia coli K 12. From a lambda transducing phage, the crp region was subcloned into pBR322. The gene was localized on the cloned fragment by determining the length of deletions which affect its expression. Its nucleotide sequence was established by using the technique of Maxam and Gilbert. The deduced amino-acid sequence is in agreement with the previously published amino acid composition of the protein (1, 2). Analysis of the sequence confirms that the DNA binding domain is located in the C-terminal portion of the protein.