Inducer-responsive expression of the cloned human interferonβ1gene introduced into cultured mouse cells | Zendy

Shigeo Ohno | Zendy; Tadatsugu Taniguchi | Zendy

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Inducer-responsive expression of the cloned human interferonβ₁gene introduced into cultured mouse cells

Author(s) -

Shigeo Ohno,

Tadatsugu Taniguchi

Publication year - 1982

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/10.3.967

Subject(s) - biology , microbiology and biotechnology , plasmid , gene , northern blot , southern blot , transfection , dna , gene expression , nucleic acid sequence , interferon , messenger rna , virology , genetics

A hybrid plasmid was constructed from pSV2-Ecogpt (Mulligan and Berg, 1981) and the cloned 1.8 kilobase chromosomal DNA segment which contains the human interferon (HIFN-beta 1) gene and its flanking sequences. Cultured mouse FM3A cells were transfected by the hybrid plasmid and several Ecogpt+ clones were selected. Southern blot analysis of the DNA from these mouse cell clones showed that two of them contained a few copies of the 1.8 kilobase human DNA. Synthesis of mRNA characteristic of the HIFN-beta 1 mRNA increased as measured by RNA blot analysis when those clones were treated with Newcastle disease virus or with poly(I):poly(C). In addition, the induced mouse cell clones gave rise to the synthesis of human IFN-beta 1. These results suggest the presence of a unique nucleotide sequence in the 1.8 kilobase DNA which is sufficient for the inducer-responsive expression of the human IFN-beta 1 gene.

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