Open AccessRearrangements of DNA-protien interactions in animal cells coupled with growth quiescence transitions
Author(s) -
A. V. Lichtenstein,
Nikolajs Sjakste,
Mikhail M. Zaboykin,
V. S. Shapot
Publication year - 1982
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/10.3.1127
Subject(s) - biology , dna , microbiology and biotechnology , genetics , biophysics , computational biology
Overall DNA-protein interactions in animal cells undergo drastic changes coupled with cellular transitions from quiescence to growth and reversely as revealed by nucleoprotein-Celite chromatography. DNA of chromatin was found to exist in one of the two sharply distinct alternative forms, namely, either tightly or weakly bound to protein moiety. These forms are specific for cycling and quiescent cells, respectively. The tight DNA-protein interactions characterize all cycling cells independent of the cell cycle phase. Transition of DNA of cycling cells from one form to another was observed as a result of treatment of isolated nuclei with DNase I.