Open AccessSequences of four tRNA genes fromCaenorhabditis elegansand the espression ofC.eleganstRNALeu(anticodon IAG) inXenopusoocytes
Author(s) -
Theresa Tranquilla,
Riccardo Cortese,
Douglas A. Melton,
John David Smith
Publication year - 1982
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/10.24.7919
Subject(s) - biology , transfer rna , xenopus , gene , caenorhabditis elegans , genetics , dna , microbiology and biotechnology , rna
Four tRNA genes have been identified in cloned segments of Caenorhabditis elegans DNA by tRNA hybridisation and expression after injection into Xenopus laevis oocyte nuclei. From DNA sequencing these are (with DNA anticodon sequences) tRNAAsp (GTC), tRNALeu (AAG), tRNALys (CTT) and tRNAPro (TGG). Their flanking DNA sequences are compared. Two identical tRNALys (CTT) genes from different regions of the genome have quite unrelated 5' flanking sequences. The tRNA synthesised in Xenopus oocytes after injection of the tRNALeu cloned DNA has the modified anticodon IAG. The tRNALeu gene precursor transcript from injected oocytes has short 5' and 3' additional sequences and lacks certain of those modified bases found in the processed tRNA.