Escherichia coliΔ1-pyrroline-5-carboxylate reductase: gene sequence, protein overproduction and purification | Zendy

Alan H. Deutch | Zendy; C. Jeffrey Smith | Zendy; Keith E. Rushlow | Zendy; P.J. Krelschmer | Zendy

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Escherichia coliΔ¹-pyrroline-5-carboxylate reductase: gene sequence, protein overproduction and purification

Author(s) -

Alan H. Deutch,

C. Jeffrey Smith,

Keith E. Rushlow,

P.J. Krelschmer

Publication year - 1982

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/10.23.7701

Subject(s) - biology , escherichia coli , microbiology and biotechnology , reductase , gene , biochemistry , plasmid , gene product , peptide sequence , overproduction , affinity chromatography , gene expression , enzyme

The sequence of the Escherichia coli proC gene which encodes for delta 1-pyrroline-5-carboxylate (PCA) reductase was determined. Overproduction of the proC gene product via an expression plasmid carrying the bacteriophage lambda PL promoter allowed the purification to homogeneity of PCA reductase by affinity adsorption chromatography. NH2 and COOH-terminal analysis and amino acid composition of the purified proC protein is consistent with the gene sequence reported. The molecular weight of the proC monomer is 28,112.

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