Open AccessEscherichia coliΔ1-pyrroline-5-carboxylate reductase: gene sequence, protein overproduction and purification
Author(s) -
A H Deutch,
C. Jeffrey Smith,
K E Rushlow,
P J Kretschmer
Publication year - 1982
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/10.23.7701
Subject(s) - biology , escherichia coli , microbiology and biotechnology , reductase , gene , biochemistry , plasmid , gene product , peptide sequence , overproduction , affinity chromatography , gene expression , enzyme
The sequence of the Escherichia coli proC gene which encodes for delta 1-pyrroline-5-carboxylate (PCA) reductase was determined. Overproduction of the proC gene product via an expression plasmid carrying the bacteriophage lambda PL promoter allowed the purification to homogeneity of PCA reductase by affinity adsorption chromatography. NH2 and COOH-terminal analysis and amino acid composition of the purified proC protein is consistent with the gene sequence reported. The molecular weight of the proC monomer is 28,112.