Open AccessOrganization and nucleotide sequence of nuclear 5S rRNA genes in yellow lupin (Lupinus lutens)
Author(s) -
Jan Antoni Rafalski,
M. Wiewiórowski,
Dieter Söll
Publication year - 1982
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/10.23.7635
Subject(s) - biology , genetics , lupinus , ribosomal rna , repeated sequence , gene , genomic dna , nucleic acid sequence , dna sequencing , dna , southern blot , restriction map , bamhi , restriction site , ribosomal dna , restriction enzyme , coding region , genomic organization , genome , phylogenetics , botany
Genomic blots of yellow lupin (Lupinus luteus) DNA digested with restriction nucleases and probed with 32P-labelled Lupinus 5S RNA reveal that 5S DNA is organized as tandemly repeated sequences of one size class, 342 bp. The DNA is extensively methylated. Two cloned BamHI ribosomal repeats were sequenced, revealing sequence divergence within both the coding and spacer regions.