The localisation of the 5′-termini ofin vivoandin vitrotranscripts of a cloned rainbow trout protamine gene | Zendy

Stephen P. Gregory | Zendy; Niall Dillon | Zendy; Peter H.W. Butterworth | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

The localisation of the 5′-termini ofin vivoandin vitrotranscripts of a cloned rainbow trout protamine gene

Author(s) -

Stephen P. Gregory,

Niall Dillon,

Peter H.W. Butterworth

Publication year - 1982

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/10.23.7581

Subject(s) - biology , protamine , rainbow trout , microbiology and biotechnology , tata box , trout , gene , transcription (linguistics) , primer extension , promoter , homology (biology) , rna , nucleic acid sequence , gene expression , genetics , biochemistry , fishery , heparin , linguistics , philosophy , fish <actinopterygii>

The mRNA start site of a cloned rainbow trout protamine gene (TPG-3) has been localised using S1-nuclease mapping and primer extension of in vivo synthesised trout testis poly A+-RNA. The presumptive cap site occurs within an AT-rich region, only 14 nucleotides from the start of the protein-coding sequence. Transcription of this protamine gene in vitro, using the Hela whole-cell extract system, generates products initiated at the same nucleotide as that used in vivo. In vitro transcription is abolished by deletion of sequences between -20 and -48, within which is a canonical TATA-box having an llbp homology with the strong chick conalbumin and Adenovirus-2 major late promoters (CTATAAAAGGG).

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research