Open AccessCharacterization of histone genes isolated fromXenopus laevisandXenopus tropicalisgenomic libraries
Author(s) -
Ida Rubati,
Paola Fragapane,
Paola Pierandrei-Amaldi,
E Beccari,
Francesco Amaldi,
Irene Bozzoni
Publication year - 1982
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/10.23.7543
Subject(s) - biology , xenopus , southern blot , gene , microbiology and biotechnology , genomic dna , genetics , complementary dna , histone , restriction map , genomic organization , genomic library , genome , nucleic acid sequence , peptide sequence
Using a cDNA clone for the histone H3 we have isolated, from two genomic libraries of Xenopus laevis and Xenopus tropicalis, clones containing four different histone gene clusters. The structural organization of X. laevis histone genes has been determined by restriction mapping, Southern blot hybridization and translation of the mRNAs which hybridize to the various restriction fragments. The arrangement of the histone genes in X. tropicalis has been determined by Southern analysis using X. laevis genomic fragments, containing individual genes, as probes. Histone genes are clustered in the genome of X. laevis and X. tropicalis and, compared to invertebrates, show a higher organization heterogeneity as demonstrated by structural analysis of the four genomic clones. In fact, the order of the genes within individual clusters is not conserved.