Silica gel: an improved support for the solid-phase phospphotriester synthesis of oligonucleotides | Zendy

Vikas Kohli | Zendy; Alain Balland | Zendy; M. Wintzerith | Zendy; R. Sauerwald | Zendy; Adrien Staub | Zendy; JeanPierre Lecocq | Zendy

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Silica gel: an improved support for the solid-phase phospphotriester synthesis of oligonucleotides

Author(s) -

Vikas Kohli,

Alain Balland,

M. Wintzerith,

R. Sauerwald,

Adrien Staub,

JeanPierre Lecocq

Publication year - 1982

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/10.22.7439

Subject(s) - oligonucleotide , oligonucleotide synthesis , biology , bromide , solid phase synthesis , combinatorial chemistry , nucleotide , silica gel , high performance liquid chromatography , chromatography , biochemistry , dna , organic chemistry , chemistry , gene , peptide

The phosphotriester method for the stepwise synthesis of deoxyoligonucleotides has been employed using HPLC-grade silica gel (Porasil B) as the solid support. The procedure results in a convenient flow-through system for the synthesis of oligomers where all the reaction steps including the zinc bromide method of detritylation are compatible with the selected support. Deoxyoligonucleotides of 25-30 nucleotides in length can be synthesized in high yields utilising stable phosphotriester intermediates. Ease of handling of the solid support allows convenient synthesis of mixed oligonucleotide sequences.

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