Open AccessTranscripts of mitochondrial tRNA genes inSaccharomyces cerevisiae
Author(s) -
Laura Frontali,
Claudio Palleschi,
Silvia Francisci
Publication year - 1982
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/10.22.7283
Subject(s) - biology , transfer rna , gene , genetics , mitochondrial dna , mutant , saccharomyces cerevisiae , nucleic acid sequence , transcription (linguistics) , dna , coding region , rna , microbiology and biotechnology , linguistics , philosophy
The transcription of a group of tRNA genes from the large tRNA gene cluster of mitochondrial DNA from Saccharomyces cerevisiae has been investigated by hybridization with DNA probes carrying tRNA coding sequences and small portions of the A + T rich intergenic regions. Results have shown that in some rho- mutants (DS502, F11) mature tRNA was absent, but a few transcripts could be detected. Some high molecular weight species actually hybridized with DNA probes carrying different tRNA coding sequences. Low molecular weight transcripts (100-150 nucleotides, carrying one tRNA sequence) were also present in these mutants. A high molecular weight transcript was also observed in the wild type, though in much more limited amount. The low molecular weight transcripts were analysed by the S1 mapping technique and found to include both a tRNA sequence and the upstream 5' flanking region extending as far as the 3' end of the preceding tRNA gene. The results suggest the existence of a common transcript bearing several tRNA sequences and indicate a possible mechanism of processing, which might be defective in mutants.